Effects of low dose rate irradiation on plateau phase bone marrow stromal cells in vitro: demonstration of a new form of non-lethal, physiologic damage to support of hematopoietic stem cells

Journal Article · Sun Jul 01 04:00:00 EDT 1984 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(84)90174-3· OSTI ID:6579340

Greenberger, J S; Klassen, V; Kase, K; Shadduck, R K; Sakakeeny, M A

The authors have developed an in vitro system for study of the effects of low dose rate (LDR) irradiation on bone marrow stromal cells. Stromal cultures were irradiated at 5 rad/min, 20 rad/min, or 200 rad/min, 24 hours or 3 weeks prior to engraftment. Two classes of irradiation damage were demonstrated following 1000 rad irradiation at 200 rad/min: 1) Decreased clonagenic survival of trypsinized replated marrow stromal cells (lethal effect); and 2) decreased production by marrow stromal cells or D2XRII cells of colony stimulating factors (CSF)s for granulocyte-macrophage progenitor cells and B6SUtA cells (physiologic effect). Holding the cultures in plateau phase for 3 weeks after irradiation was associated with significantly more repair of the lethal effect compared to the physiologic effect. These data demonstrate a distinct physiologic expression of irradiation damage to bone marrow stromal cells that affects cell to cell interaction, responds differently to changes in dose rate, and is repaired with kinetics different from those of the lethal effect of irradiation. The present system should prove valuable for investigation of cellular interactions in hematopoietic stem cell engraftment that are altered by total body irradiation.

• Research Organization: Harvard Medical School, Cambridge, MA
• OSTI ID: 6579340
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Vol. 10:7; ISSN IOBPD
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BONE MARROW CELLS
CELL KILLING
COLONY FORMATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
IN VITRO
IONIZING RADIATIONS
IRRADIATION
ISOTOPE APPLICATIONS
LOW DOSE IRRADIATION
MAMMALS
MICE
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOINDUCTION
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
STEM CELLS
TRACER TECHNIQUES
VERTEBRATES
X RADIATION