Genetics and biochemistry of surfactant synthesis in Arthrobacter species H-13A: Progress report, (August 1, 1986 through March 31, 1987)

Technical Report · Thu Jan 01 00:00:00 EST 1987

OSTI ID:6577357

Finnerty, W R

The major objectives of this study have been to develop a genetic transfer system and a molecular cloning technology in Arthrobacter sp. H13A in order to isolate the genes encoding glycolipid biosynthesis and to study their genetic organization and regulation. Our preliminary findings show the development of a plasmid transformation system in H13-A and show the development of a plasmid transformation system in H13-A and an E. coli - Arthrobacter shuttle vector. In this report, we describe the optimization of plasmid transformation in H13-A, the molecular characterization of the shuttle vector, including a restriction map, stability and host range of the plasmid and the expression of the plasmid-borne antibiotic resistance markers in H13-A. 6 refs., 3 tabs.

• Research Organization: Georgia Univ., Athens (USA). Dept. of Microbiology
• DOE Contract Number: FG09-86ER13588
• OSTI ID: 6577357
• Report Number(s): DOE/ER/13588-1; ON: DE87009037
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOLIPIDS
BIOLOGICAL PATHWAYS
RECOMBINANT DNA
SURFACTANTS
BIOSYNTHESIS
BACTERIA
GENETIC ENGINEERING
PLASMIDS
CARBOHYDRATES
CELL CONSTITUENTS
DNA
LIPIDS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SACCHARIDES
SYNTHESIS
550200* - Biochemistry
550500 - Metabolism
550700 - Microbiology