Effects of 2,2,2-trifluoroethanol on the testis of the rat

Technical Report · Thu Jan 01 04:00:00 EST 1981

OSTI ID:6577035

Wilkenfeld, R M

The effects of xenobiotics on the male reproductive system have become a growing concern. During an investigation into the acute toxicity of the volatile solvent 2,2,2-trifluoroethanol (TFE) following inhalation, it was noted that testicular damage was a consistent effect. Single intraperitoneal doses of TFE as low as 25 mg/kg produced damage to spermatogonia and spermatocytes, but had no effect on postmeiotic germ cells or interstitial structures. Damage to the testis was rapid, first being detectable by light microscopy 8 hours following a single i.p. dose. In order to determine the effects of subchronic inhalation exposre on male reproductive function, rats were exposed to trifluoroethanol at concentrations of 0, 100 ppM and 200 ppM, for 6 hours/day, 5 days/week for two weeks. The absorption, distribution, and excretion of intraperitoneally injected trifluoroethanol (5 mg/kg and 100 mg/kg was studied using 2-/sup 14/C-TFE. Testicular and epididymal blood flow, as determined using 15 ..mu..m /sup 57/Co-microspheres, was not altered 15 minutes, 1, 3, or 6 hours following 100 mg TFE/kg. In vivo protein incorporation of /sup 14/C-leucine in the testis was not altered 6 hours after TFE. It appears that TFE-induced testicular damage is not due to decreased blood flow, as has been suggested for cadmium-induced damage. Alteration of the redox state of the testis may be suggested by the decrease in lactate concentration. 114 references, 16 figures, 25 tables.

• Research Organization: Rochester Univ., NY (USA). School of Medicine and Dentistry
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 6577035
• Report Number(s): DOE/EV/03490-2436; ON: DE84016273
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ALCOHOLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBON 14
CARBON ISOTOPES
CHRONIC EXPOSURE
COBALT 57
COBALT ISOTOPES
DATA
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ETHANOL
EVEN-EVEN NUCLEI
EXPERIMENTAL DATA
FLUORINATED ALIPHATIC HYDROCARBONS
GONADS
HALOGENATED ALIPHATIC HYDROCARBONS
HISTOLOGY
HYDROXY COMPOUNDS
INFORMATION
INHALATION
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAPERITONEAL INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MALE GENITALS
MAMMALS
METABOLISM
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANS
RADIOISOTOPES
RATS
REPRODUCTION
RODENTS
SOLVENTS
TESTES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES
YEARS LIVING RADIOISOTOPES