Localization of the interferon-induced, 2-5A-dependent RNase gene (RNS4) to human chromosome 1q25

Journal Article · Sat Jan 01 04:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1033· OSTI ID:6573877

Squire, J ^[1]; Zhou, A; Hassel, B A; Nie, H; Silverman, R H ^[2]

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1. Hospital for Sick Children, Ontario (Canada)
2. Cleveland Clinic Foundation, OH (United States)

2-5A-dependent RNase is a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative activities of interferons. Interferon treatment of cells results in enhanced levels of both 2-5A-dependent RNase and a group of synthetases that produce 5[prime]-triphosphorylated, 2[prime],5[prime]-oligoadenylates (2-5A) from ATP. The 2-5A-synthetases are stimulated by double-stranded RNA (dsRNA), a frequent product of virus infection. 2-5A activates 2-5A-dependent RNase, resulting in the cleavage of viral and cellular single-stranded RNAs 3[prime] of UpAp and UpUp. Levels of 2-5A-dependent RNase and/or 2-5A-synthetase vary with growth conditions, hormone status, liver regeneration, and cell differentiation, thus suggesting a possible wider role for the 2-5A system in the general control of RNA decay. The recent molecular cloning of the human and murine forms of 2-5A-dependent RNase revealed a repeated phosphate binding loop motif, homology with protein kinases, zinc fingers, and Escherichia coli RNase E, and the presence of nine ankyrin repeats implicated in mediating protein-protein interactions. The role of the 2-5A system in the control of viral and cellular growth suggests that defects in the 2-5A-dependent RNase (RNS4) gene could result in reduced immunity to virus infections and cancer, thus underscoring the importance of determining the position of the RNS4 gene in the human genome. Here, the authors have localized the human gene for 2-5A-dependent RNase (RNS4) by fluorescence in situ hybridization to chromosome 1q25. The regional assignment of the RNS4 gene was determined by fluorescence in situ hybridization following published methods, using a genomic 2-5A-dependent RNase clone previously isolated from a human placenta cosmid library in vector pWE15. 15 refs., 1 fig.

• OSTI ID: 6573877
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:1; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOTECHNOLOGY
CHROMOSOMES
CLONING
DNA HYBRIDIZATION
DNA-CLONING
ENZYMES
ESTERASES
GENES
GENETIC ENGINEERING
GENETIC MAPPING
HUMAN CHROMOSOME 1
HUMAN CHROMOSOMES
HYBRIDIZATION
HYDROLASES
MAPPING
NUCLEIC ACID HYBRIDIZATION
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
RNA-ASE