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Simultaneous determination of free thyroxine and capacity of thyroxine-binding globulin

Journal Article · · J. Nucl. Med.; (United States)
OSTI ID:6568970
; ; ;
A simple method is described for the simultaneous determination of capacity thyroxine-binding of globulin (TBG) and free thyroxine concentration (FT/sub 4/). The ratio of bound to free T/sub 4/ (B/F) is first determined for two total-T/sub 4/ concentrations using a Sephadex G-25 competitive-binding technique. TBG capacity and FT/sub 4/ can both be calculated assuming a known value of affinity constant of TBG. The method is linear over a twenty-fold serum dilution. FT/sub 4/ calculated is identical to that calculated using the method of Irvine. TBG capacity is shown to be linearly correlated to TBG concentration as determined by radioimmunoassay and is consistent with a molecular weight of 69,000 Daltons and one T/sub 4/ binding site per molecule. FT/sub 4/ is found to correlate with the free thyroxine index in a complicated way, depending on the degree of TBG saturation.
Research Organization:
Univ. of California, Los Angeles
OSTI ID:
6568970
Journal Information:
J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 22:3; ISSN JNMEA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
CALCULATION METHODS
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHEMISTRY
CHROMATOGRAPHY
CPB
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
PATIENTS
PEPTIDE HORMONES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
SEPARATION PROCESSES
THYROID HORMONES
THYROXINE
TRACER TECHNIQUES