Direct assays of radiation-induced DNA base lesions in mammalian cells: Technical progress report, July 1, 1986--December 1, 1988

Technical Report · Fri Jan 01 04:00:00 EST 1988

OSTI ID:6555443

Wheeler, Jr, K T

Our cesium irradiator was installed in April 1987 and has the capability of irradiating DNA solutions, cells and animals at dose rates from >60 Gy/min to <10/sup /minus/2/ Gy/min. By early summer all of the dosimetry and set-ups were established to perform this research. In may 1987, Dr. Krystyna Lesiak left to return to the National Institutes of Health. However, she has remained a collaborator over the past 1/1/2/ years. She has synthesized a large lot of ..cap alpha..-deoxyadenosine, isolated a large batch of both the R and S isomer of cyclodeoxyadenosine and has capped the ..cap alpha..-deoxyadenonsine for use in a DNA synthesizer that uses phosphoramidite chemistry. In November 1987, Dr. Andrzej Surowiec joined our unit as a Visiting Research Assistant Professor. Dr. Surowiec has a MS degree in electrical engineering and did his Ph.D. in Biophysics studying the conductivity of DNA in dilute solution. He has been performing the helix-coil transition experiments. In November 1987, Dr. Steven Swarts also joined our unit as a Postdoctoral Fellow. He received his Ph.D. from Oakland University under Dr. Michael Sevilla with whom we have a collaboration studying the induction of base damage in hydrated DNA. Dr. Swarts has a strong background in spectroscopy and, therefore, was a key individual for determination of the limitations of the HPLC assays and the establishment of a GC/MS capability equivalent to Dr. M. Dizdaroglu at the National Bureau of Standards. 9 refs., 1 tab.

• Research Organization: Bowman Gray School of Medicine, Winston-Salem, NC (USA). Dept. of Radiology
• DOE Contract Number: FG05-86ER60464
• OSTI ID: 6555443
• Report Number(s): DOE/ER/60464-3; ON: DE89005386
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
&amp; Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CARCINOMAS
CELL CYCLE
CELL PROLIFERATION
DISEASES
DNA REPAIR
DOCUMENT TYPES
NEOPLASMS
PROGRESS REPORT
RADIOSENSITIVITY
RECOVERY
REPAIR
RESPONSE MODIFYING FACTORS
TUMOR CELLS