The L-isoaspartyl/D-aspartyl protein methyltransferase gene (PCMT1) maps to human chromosome 6q22. 3-6. q24 and the syntenic region of mouse chromosome 10

Journal Article · Tue Dec 01 04:00:00 EST 1992 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80104-1· OSTI ID:6553757

MacLaren, D C; O'Conner, C M ^[1]; Xia, Yu-Rong; Mehrabian, M; Klisak, I; Sparkes, R S; Lusis, A J ^[2]; Clarke, S

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1. Worcester Foundation for Experimental Biology, Shrewsbury, MA (United States)
2. Univ. of California, Los Angeles, CA (United States)

The authors have mapped the genes for the human and mouse L-isoaspartyl./D-aspartyl protein carboxyl methyltransferase (EC 2.1.1.77) using cDNA probes. They determined that the human gene is present in chromosome 6 by Southern blot analysis of DNA from a panel of mouse-human somatic cell hybrids. In situ hybridization studies allowed confirmation of this identification and further localized the human gene (PCMT1) to the 6q22.3-6q24 region. By analyzing the presence of an EcoRI polymorphism in DNA from backcrosses of C57BL/6J and Mus spretus strains of mice, they localized the mouse gene (Pcmt-1) to chromosome 10, at a position 8.2 [+-] 3.5 cM proximal to the Myb locus. This region of the mouse chromosome is homologous to the human 6q24 region. 32 refs., 2 figs., 2 tabs.

• OSTI ID: 6553757
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 14:4; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ASPARTIC ACID
BIOTECHNOLOGY
CARBON-GROUP TRANSFERASES
CARBOXYLIC ACIDS
CHROMOSOMES
DNA HYBRIDIZATION
ENZYMES
GENES
GENETIC ENGINEERING
GENETIC MAPPING
HUMAN CHROMOSOME 6
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
METHYL TRANSFERASES
NUCLEIC ACID HYBRIDIZATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RFLPS
TRANSFERASES