Detection of retinoblastoma gene deletions in spontaneous and radiation-induced mouse lung adenocarcinomas by polymerase chain reaction

Journal Article · Tue Mar 01 04:00:00 EST 1994 · Radiation Research; (United States)

DOI:https://doi.org/10.2307/3578704· OSTI ID:6551377

Churchill, M E; Gemmell, M A; Woloschak, G E ^[1]

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1. Argonne National Lab., IL (United States)

A polymerase chain reaction (PCR) technique has been developed to detect deletions in the mouse retinoblastoma gene using histological sections from radiation-induced and spontaneous tumors as the DNA source. Six mouse Rb gene exon fragments were amplified in a 40-cycle, 3-temperature PCR protocol. The absence of any of these fragments relative to control PCR products on a Southern blot indicated a deletion of that portion of the mouse Rb gene. Tumors chosen for analysis were lung adenocarcinomas that were judged to be the cause of death. Spontaneous tumors as well as those from irradiated mice (5.69 Gy [sup 60]Co [gamma] rays or 0.6 Gy JANUS neutrons, which have been found to have approximately equal radiobiological effectiveness) were analyzed for mouse Rb deletions. Tumors in 6 neutron-irradiated mice had no mouse Rb deletions. However, 1 of 6 tumors from [gamma]-irradiated mice (17%) and 6 of 18 spontaneous tumors from unirradiated mice (33%) showed a deletion in one or both mouse Rb alleles. All deletions detected were in the 5[prime] region of the mouse Rb gene. 36 refs., 2 figs., 2 tabs.

• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6551377
• Journal Information: Radiation Research; (United States), Journal Name: Radiation Research; (United States) Vol. 137:3; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300 -- Cytology
560150* -- Radiation Effects on Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BEAMS
BIOTECHNOLOGY
CHROMOSOMAL ABERRATIONS
DETECTION
DISEASES
ELECTROMAGNETIC RADIATION
FEASIBILITY STUDIES
GAMMA RADIATION
GENE AMPLIFICATION
GENETIC ENGINEERING
IONIZING RADIATIONS
MAMMALS
MICE
MUTATIONS
NEOPLASMS
NEUTRON BEAMS
NUCLEON BEAMS
PARTICLE BEAMS
POLYMERASE CHAIN REACTION
RADIATIONS
RADIOINDUCTION
RODENTS
VERTEBRATES