DNA repair in a short- and a long-lived rodent species

Journal Article · Mon Jan 01 04:00:00 EST 1979 · J. Gerontol.; (United States)

DOI:https://doi.org/10.1093/geronj/34.6.808· OSTI ID:6551347

Hart, R W ^[1]; Sacher, G A; Hoskins, T L

---

1. Ohio State Univ., Columbus

Previous studies have reported a direct correlation between the rate of uv-induced unscheduled DNA synthesis and the maximum lifespan for seven mammalian species drawn from five different orders. We now examine this correlation in laboratory populations of two rodent species, the house mouse, Mus musculus, and the white-footed mouse, Peromyscus leucopus, both in the superfamily Muroidea. These species are similar in body size but Peromyscus exceeds Mus in lifespan by a factor of 2.5, as determined by live table studies in the same laboratory environment. The extent of uv-induced unscheduled DNA synthesis by third-passage primary fibroblast cell cultures was measured for these two species, and the rate of DNA synthesis was 2.5 times greater for the Peromyscus cells, consistent with the greater lifespan of Peromyscus and with the relationship of repair of uv damage to lifespan found previously among representatives of five orders of mammals. A determination of patch size (size of the repair regions) by bromouracil photolysis following ultraviolet irradiation revealed that the patch sizes were the same for the two species, and that the number of repaired sites was 2.2 times greater for Peromyscus than for Mus, in agreement with the ratio found by measurement of unscheduled DNA synthesis. The number of single strand breaks induced by gamma rays, and the rate of rejoining of these breaks, as measured on alkaline sucrose gradients, were not significantly different in the two species.

• OSTI ID: 6551347
• Journal Information: J. Gerontol.; (United States), Journal Name: J. Gerontol.; (United States) Vol. 34:6; ISSN JOGEA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560114* -- Radiation Effects on Biochemicals-- In Animals-- (-1987)
560152 -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL VARIABILITY
BIOSYNTHESIS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DNA
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GAMMA RADIATION
IONIZING RADIATIONS
LIFE SPAN
MAMMALS
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
STRAND BREAKS
SYNTHESIS
ULTRAVIOLET RADIATION
VERTEBRATES