Secretion of a malarial histidine-rich protein (Pf HRP II) from Plasmodium falciparum-infected erythrocytes
Plasmodium falciparum-infected erythrocytes (IRBCs) synthesis several histidine-rich proteins (HRPs) that accumulate high levels of (/sup 3/H)histidine but very low levels of amino acids such as (/sup 3/H)isoleucine or (/sup 35/S)methionine. The authors prepared a monoclonal antibody which reacts specifically with one of these HRPs (Pf HRP II) and studied the location and synthesis of this protein during the parasite's intracellular growth. With the knob-positive Malayan Camp strain of P. falciparum, the monoclonal antibody identified a multiplet of protein of protein bands with major species at M/sub r/ 72,000 and 69,000. Pf HRP II synthesis began with immature parasites (rings) and continued through the trophozoite stage. The M/sub r/ 72,000 band of Pf HRP II, but not the faster moving bands of the multiplet, was recovered as a water-soluble protein from the culture supernatant of intact IRBCs. Approximately 50% of the total (/sup 3/H)histidine radioactivity incorporated into the M/sub r/ 72,000 band was extracellular between 2 and 24 h of culture. Immunofluorescence and cryothin-section immunoelectron microscopy localized Pf HRP II to several cell compartments including the parasite cytoplasm, as concentrated packets in the host erythrocyte cytoplasm and at the IRBC membrane. The results provide evidence for an intracellular route of transport for a secreted malarial protein from the parasite through several membranes and the host cell cytoplasm.
- Research Organization:
- National Institutes of Health (NIH), Bethesda, MD (United States)
- OSTI ID:
- 6548926
- Journal Information:
- J. Cell Biol.; (United States), Vol. 103:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
BIOSYNTHESIS
SECRETION
SULFUR 35
SUBCELLULAR DISTRIBUTION
TRITIUM COMPOUNDS
ELECTRON MICROSCOPY
ELECTROPHORESIS
ERYTHROCYTES
HISTIDINE
METHIONINE
MONOCLONAL ANTIBODIES
PLASMODIUM
AMINO ACIDS
ANIMALS
ANTIBODIES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
INVERTEBRATES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
MICROORGANISMS
MICROSCOPY
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PROTOZOA
RADIOISOTOPES
SPOROZOA
SULFUR ISOTOPES
SYNTHESIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics