Secretion of a malarial histidine-rich protein (Pf HRP II) from Plasmodium falciparum-infected erythrocytes

Journal Article · Wed Oct 01 04:00:00 EDT 1986 · J. Cell Biol.; (United States)

DOI:https://doi.org/10.1083/jcb.103.4.1269· OSTI ID:6548926

Howard, R J; Uni, S; Aikawa, M; Aley, S B; Leech, J H; Lew, A M; Wellems, T E; Rener, J; Taylor, D W

Plasmodium falciparum-infected erythrocytes (IRBCs) synthesis several histidine-rich proteins (HRPs) that accumulate high levels of (/sup 3/H)histidine but very low levels of amino acids such as (/sup 3/H)isoleucine or (/sup 35/S)methionine. The authors prepared a monoclonal antibody which reacts specifically with one of these HRPs (Pf HRP II) and studied the location and synthesis of this protein during the parasite's intracellular growth. With the knob-positive Malayan Camp strain of P. falciparum, the monoclonal antibody identified a multiplet of protein of protein bands with major species at M/sub r/ 72,000 and 69,000. Pf HRP II synthesis began with immature parasites (rings) and continued through the trophozoite stage. The M/sub r/ 72,000 band of Pf HRP II, but not the faster moving bands of the multiplet, was recovered as a water-soluble protein from the culture supernatant of intact IRBCs. Approximately 50% of the total (/sup 3/H)histidine radioactivity incorporated into the M/sub r/ 72,000 band was extracellular between 2 and 24 h of culture. Immunofluorescence and cryothin-section immunoelectron microscopy localized Pf HRP II to several cell compartments including the parasite cytoplasm, as concentrated packets in the host erythrocyte cytoplasm and at the IRBC membrane. The results provide evidence for an intracellular route of transport for a secreted malarial protein from the parasite through several membranes and the host cell cytoplasm.

• Research Organization: National Institutes of Health, Bethesda, MD
• OSTI ID: 6548926
• Journal Information: J. Cell Biol.; (United States), Journal Name: J. Cell Biol.; (United States) Vol. 103:4; ISSN JCLBA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACIDS
ANIMALS
ANTIBODIES
AZOLES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
DRUGS
ELECTRON MICROSCOPY
ELECTROPHORESIS
ERYTHROCYTES
EVEN-ODD NUCLEI
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
INVERTEBRATES
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
LIPOTROPIC FACTORS
MATERIALS
METHIONINE
MICROORGANISMS
MICROSCOPY
MONOCLONAL ANTIBODIES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PARASITES
PLASMODIUM
PROTEINS
PROTOZOA
RADIOISOTOPES
SECRETION
SPOROZOA
SUBCELLULAR DISTRIBUTION
SULFUR 35
SULFUR ISOTOPES
SYNTHESIS
TRITIUM COMPOUNDS