Extraction and purification of calcium-activated photoproteins from the ctenophores Mnemiopsis sp. and Beroe ovata

Journal Article · Tue Jan 01 04:00:00 EST 1974 · Biochemistry; (United States)

OSTI ID:6543452

Ward, W W; Seliger, H H

Calcium-activated bioluminescent ''photoproteins'' from the ctenophores Mnemiopsis sp. and Beroue ovata have been isolated by extraction with EDTA and purified 3800-fold and 7800-fold, respectively. The steps consisted of ammonium sulfate and protoamine sulfate treatments, followed by several steps of ion-exchange, gel filtration, and solubility chromatography. The purified photoproteins are virtually homogeneous by the criterion of sodium dodecyl sulfate polyacrylamide gel electrophoresis. Approximately 30,000 adult specimens of Mnemiopsis, totalling more than 600 kg wet wt, yielded 2 mg of purified photoprotein. Mnemiopsis photoprotein has been resolved into two functionally identical forms (''isoproteins''), termed mnemiopsin-1 and mnemiopsin-2, by chromatography on DEAE-cellulose. Berovin, the Beroue photoprotein, chromatographs as a single symmetrical peak under the same conditions.

• Research Organization: Johns Hopkins Univ., Baltimore
• OSTI ID: 6543452
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 13:7; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AMINO ACIDS
AMMONIUM COMPOUNDS
AMMONIUM SULFATES
ANIMALS
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CALCIUM
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMATOGRAPHY
CNIDARIA
COMPARATIVE EVALUATIONS
EDTA
ELECTROPHORESIS
ELEMENTS
EMISSION
EXTRACTION
INVERTEBRATES
ION EXCHANGE
KINETICS
LUMINESCENCE
METALS
OPTICAL PROPERTIES
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOTOLUMINESCENCE
PHOTON EMISSION
PHYSICAL PROPERTIES
PROTEINS
PURIFICATION
REACTION KINETICS
SEPARATION PROCESSES
SULFATES
SULFUR COMPOUNDS