Neuroblastoma membranes inhibit isoproterenol-stimulated rise of cAMP in glioma cells. [Rats]
C6 glioma cells respond to ..beta..-adrenergic agonists (isoproterenol) with a transient rise in intracellular cyclic adenosine monophosphate level. This ..beta..-responsiveness of C6 cells is inhibitied by the presence of a plasma membrane fraction, which has a five- to six-fold purification of membrane markers, showed a greater inhibition of ..beta..-responsiveness in C6 cells than any other subcellular fractions of B104 cells. The inhibitory effector(s) is apparently associated with integral membrane structure(s) since ionic extraction and treatment with chelating agents did not remove the effect from the particulate membrane fraction. The effector is probably proteinaceous in nature as judged by its susceptibility to inactivation by heat and protease treatment. The data indicate that neither adenylate cyclase nor phosphodiesterase enzyme is likely to be directly involved in mediating the ..beta..-nonresponsiveness of C6 cells.
- Research Organization:
- Univ. of California, Los Angeles
- DOE Contract Number:
- AC03-76SF00012
- OSTI ID:
- 6541894
- Journal Information:
- J. Cell. Physiol.; (United States), Vol. 118:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMP
INHIBITION
CELL MEMBRANES
PHYSIOLOGY
SYMPATHOMIMETICS
BIOCHEMICAL REACTION KINETICS
ANIMAL CELLS
CELL CULTURES
ENZYME ACTIVITY
GLIOMAS
SARCOMAS
AUTONOMIC NERVOUS SYSTEM AGENTS
CELL CONSTITUENTS
DISEASES
DRUGS
KINETICS
MEMBRANES
NEOPLASMS
NUCLEOTIDES
ORGANIC COMPOUNDS
REACTION KINETICS
550200* - Biochemistry