New sensitive assay for the measurement of hydroperoxides
Book
·
OSTI ID:6541857
The enzyme glutathione (GSH) peroxidase can be used to measure hydroperoxides quantitatively, easily, and specifically. A timed reaction of GSH peroxidase, coupled with the oxidation of NADPH by GSH reductase, allows a direct spectrophotometric measurement of hydroperoxide. Addition of catalase prior to the addition of GSH peroxidase permits the distinction between hydrogen peroxide and organic hydroperoxides. The solvents that can be used with the assay include methanol, ethanol, water, and aqueous solutions of detergents such as Brij 35, Triton X-100, and cetyl trimethyl ammonium bromide. The utility of the method is demonstrated by the measurement of hydrogen peroxide and organic hydroperoxides formed upon ozonolysis of an unsaturated fatty acid.
- OSTI ID:
- 6541857
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BIOASSAY
CARBOXYLIC ACIDS
CATALASE
DATA
DRUGS
ENZYME ACTIVITY
ENZYMES
ETHANOL
EXPERIMENTAL DATA
GLUTATHIONE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROPEROXY RADICALS
HYDROXY COMPOUNDS
INFORMATION
MEASURING METHODS
METHANOL
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDASES
PEROXIDES
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADICALS
RADIOPROTECTIVE SUBSTANCES
59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BIOASSAY
CARBOXYLIC ACIDS
CATALASE
DATA
DRUGS
ENZYME ACTIVITY
ENZYMES
ETHANOL
EXPERIMENTAL DATA
GLUTATHIONE
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
HYDROPEROXY RADICALS
HYDROXY COMPOUNDS
INFORMATION
MEASURING METHODS
METHANOL
NUCLEOTIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDASES
PEROXIDES
POLYPEPTIDES
PORPHYRINS
PROTEINS
RADICALS
RADIOPROTECTIVE SUBSTANCES