Effect of chromosomal homology on plasmid transfer and transformation in Streptococcus pneumoniae
The obvious rarity of simultanaeous uptake of two recombinant plasmids bearing the same chromosomal segment militated against the likelihood of cloning such segments by the conventional approach. However, it was possible to clone chromosomal genes in S. pneumoniae, and clones of two well-studied pneumococcal genes, malM, which codes for the amylomaltase essential for maltose utilization, and sul-d, which confers sulfonamide resistance, were obtained. The availability of recombinant plasmids carrying DNA segments homologous to the chromosome allowed investigation of the effects of such homology on plasmid establishment. In addition to facilitation of plasmid establishment, evidence was obtained for a subsequent process, in which alleles were equilibrated between the chromosome and the plasmid pool.
- Research Organization:
- Brookhaven National Lab., Upton, NY
- OSTI ID:
- 6540120
- Journal Information:
- Microbiology (Engl. Transl.); (United States), Journal Name: Microbiology (Engl. Transl.); (United States); ISSN MIBLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AMIDES
ANTI-INFECTIVE AGENTS
ANTIMICROBIAL AGENTS
BACTERIA
BIOLOGY
CARBOHYDRATES
CELL CONSTITUENTS
CLONING
DISACCHARIDES
DNA
DNA-CLONING
DRUGS
ENZYMES
GENETIC MAPPING
GENETICS
GLYCOSYL HYDROLASES
HYDROLASES
MALTOSE
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLASMIDS
RECOMBINANT DNA
SACCHARIDES
STREPTOCOCCUS
SULFONAMIDES