Fermentation of soluble cello-oligosaccharides by yeasts
Yeast strains that ferment cellobiose were examined with respect to fermentation on soluble cellodextrin preparations. Hydrolysis of the fermentation products was followed using thin layer chromatography. Candida and Brettanomyces sp. hydrolyze cellobiose and, at a much lower rate, cellotriose, indicating the presence of ..beta..-glucosidase (EC 3.2.1.21) activity. Enzyme assays conducted on B. clausenii fermentations indicated that the ..beta..-glucosidase remained cell-associated during fermentation. Torulopsis sp. hydrolyzed all of the cello-oligo-saccharides, indicating exoglucanase (EC 3.2.1.91) activity. The exogluconanase, a glycoprotein with an apparent molecular weight of 8.4 x 10/sup 4/ daltons, is exported into the culture medium.
- Research Organization:
- Solar Energy Research Inst., Golden, CO (USA)
- DOE Contract Number:
- AC02-77CH00178
- OSTI ID:
- 6538777
- Report Number(s):
- SERI/TP-232-1733; CONF-820909-18; ON: DE83007317
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
BIOCONVERSION
CANDIDA
CARBOHYDRATES
CELLOBIOSE
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
FERMENTATION
FUNGI
HYDROLYSIS
LYSIS
MICROORGANISMS
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TORULA
YEASTS