Fermentation of soluble cello-oligosaccharides by yeasts

Conference · Tue Feb 01 00:00:00 EST 1983

OSTI ID:6538777

Lastick, S M; Spindler, D D; Grohmann, K

Yeast strains that ferment cellobiose were examined with respect to fermentation on soluble cellodextrin preparations. Hydrolysis of the fermentation products was followed using thin layer chromatography. Candida and Brettanomyces sp. hydrolyze cellobiose and, at a much lower rate, cellotriose, indicating the presence of ..beta..-glucosidase (EC 3.2.1.21) activity. Enzyme assays conducted on B. clausenii fermentations indicated that the ..beta..-glucosidase remained cell-associated during fermentation. Torulopsis sp. hydrolyzed all of the cello-oligo-saccharides, indicating exoglucanase (EC 3.2.1.91) activity. The exogluconanase, a glycoprotein with an apparent molecular weight of 8.4 x 10/sup 4/ daltons, is exported into the culture medium.

• Research Organization: Solar Energy Research Inst., Golden, CO (USA)
• DOE Contract Number: AC02-77CH00178
• OSTI ID: 6538777
• Report Number(s): SERI/TP-232-1733; CONF-820909-18; ON: DE83007317
• Resource Relation: Conference: National meeting of the American Chemical Society, Kansas City, MO, USA, 1 Sep 1982
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
OLIGOSACCHARIDES
FERMENTATION
CANDIDA
CELLOBIOSE
CELLULOSE
HYDROLYSIS
TORULA
YEASTS
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
DISACCHARIDES
FUNGI
LYSIS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
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