Opioid-dependent growth of glial cultures: Suppression of astrocyte DNA synthesis by met-enkephalin
Journal Article
·
· Life Sciences; (USA)
- Univ. of Kentucky, Lexington (USA)
The action of met-enkephalin on the growth of astrocytes in mixed-glial cultures was examined. Primary, mixed-glial cultures were isolated from 1 day-old mouse cerebral hemispheres and continuously treated with either basal growth media, 1 {mu}M met-enkephalin, 1 {mu}M met-enkephalin plus the opioid antagonist naloxone, or naloxone alone. Absolute numbers of neural cells were counted in unstained preparations, while combined ({sup 3}H)-thymidine autoradiography and glial fibrillary acid protein (GFAP) immunocytochemistry was performed to identify specific changes in astrocytes. When compared to control and naloxone treated cultures, met-enkephalin caused a significant decrease in both total cell numbers, and in ({sup 3}H)-thymidine incorporation by GFAP-positive cells with flat morphology. These results indicate that met-enkephalin suppresses astrocyte growth in culture.
- OSTI ID:
- 6532939
- Journal Information:
- Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 46:2; ISSN 0024-3205; ISSN LIFSA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DNA REPLICATION
DRUGS
ENDORPHINS
ENKEPHALINS
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INHIBITION
MAMMALS
MICE
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DNA REPLICATION
DRUGS
ENDORPHINS
ENKEPHALINS
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INHIBITION
MAMMALS
MICE
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES