Effects of lithium chloride on testicular steroidogenic and gametogenic functions in mature male albino rats

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90045-S· OSTI ID:6532922

Ghosh, D; Chaudhuri, A; Biswas, N M; Ghosh, P K ^[1]

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1. Calcutta Univ. (India)

The present study was undertaken to evaluate the effects of lithium, on steroidogenic and gametogenic functions of testis in the rat. Adult male rats of Wistar strain were injected with lithium chloride at the dose of 0.1 mg, 0.2 mg, and 0.4 mg/100 g body weight/day for 21 days. All the treated animals along with the vehicle treated controls were sacrificed 24 hours after the last injections. Testicular steroidogenic activity was evaluated by measuring the activities of two steroidogenic key enzymes, {Delta}{sup 5}-3{beta} hydroxysteriod dehydrogenase ({Delta}{sup 5} -3{beta}-HSD) and 17{beta} hydroxysteroid dehydrogenase (17{beta} -HSD). Gametogenic capacity was determined by counting the number of germ cells at stage VII of seminiferous cycle. Plasma levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and testosterone (T) were measured by radioimmunoassay (RIA). Administration of lithium chloride at a dose of 0.1 mg/100g body wt. for 21 days led to insignificant changes of plasma FSH, LH, PRL and T along with unaltered activities of testicular {Delta}5 -3{beta}-HSD, 17 {beta}-HSD activities and gametogenesis.

• OSTI ID: 6532922
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 46:2; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALI METAL COMPOUNDS
ANDROGENS
ANDROSTANES
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CHLORIDES
CHLORINE COMPOUNDS
DIAGNOSTIC TECHNIQUES
ENZYME ACTIVITY
ENZYMES
FSH
FUNCTIONS
GONADOTROPINS
GONADS
HALIDES
HALOGEN COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
LH
LITHIUM CHLORIDES
LITHIUM COMPOUNDS
LITHIUM HALIDES
LTH
MALE GENITALS
MALES
MAMMALS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PITUITARY HORMONES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RATS
RODENTS
STEROID HORMONES
STEROIDS
TESTES
TESTOSTERONE
TRACER TECHNIQUES
VERTEBRATES