ATP-dependent and NAD-dependent modification of glutamine synthetase from Rhodospirillum rubrum in vitro

Journal Article · Wed Aug 15 04:00:00 EDT 1990 · Journal of Biological Chemistry; (USA)

OSTI ID:6532140

Woehle, D L; Lueddecke, B A; Ludden, P W ^[1]

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1. Univ. of Wisconsin, Madison (USA)

Glutamine synthetase from the photosynthetic bacterium Rhodospirillum rubrum is the target of both ATP- and NAD-dependent modification. Incubation of R. rubrum cell supernatant with ({alpha}-{sup 32}P)NAD results in the labeling of glutamine synthetase and two other unidentified proteins. Dinitrogenase reductase ADP-ribosyltransferase does not appear to be responsible for the modification of glutamine synthetase or the unidentified proteins. The ({alpha}-{sup 32}P)ATP- and ({alpha}-{sup 32}P) NAD-dependent modifications of R. rubrum glutamine synthetase appear to be exclusive and the two forms of modified glutamine synthetase are separable on two-dimensional gels. Loss of enzymatic activity by glutamine synthetase did not correlate with ({alpha}-{sup 32}P)NAD labeling. This is in contrast to inactivation by nonphysiological ADP-ribosylation of other glutamine synthetases by an NAD:arginine ADP-ribosyltransferase from turkey erythrocytes. A {sup 32}P-labeled protein spot comigrates with the NAD-treated glutamine synthetase spot when glutamine synthetase purified from H{sub 3} {sup 32}PO{sub 4}-grown cells is analyzed on two-dimensional gels. The adenylylation site of R. rubrum glutamine synthetase has been determined to be Leu-(Asp)-Tyr-Leu-Pro-Pro-Glu-Glu-Leu-Met; the tyrosine residue is the site of modification.

• OSTI ID: 6532140
• Journal Information: Journal of Biological Chemistry; (USA), Journal Name: Journal of Biological Chemistry; (USA) Vol. 265:23; ISSN JBCHA; ISSN 0021-9258
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADP
ALDEHYDES
AMIDES
AMINO ACID SEQUENCE
AMINO ACIDS
ATP
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CARBOXYLIC ACIDS
COENZYMES
DAYS LIVING RADIOISOTOPES
ELECTROPHORESIS
ENZYME ACTIVITY
ENZYMES
GLUTAMINE
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLING
LIGASES
LIGHT NUCLEI
MEMBRANE PROTEINS
MICROORGANISMS
MOLECULAR STRUCTURE
MONOSACCHARIDES
NAD
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PENTOSES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RHODOSPIRILLUM
RIBOSE
SACCHARIDES
TRACER TECHNIQUES
TWO-DIMENSIONAL ELECTROPHORESIS