Casein gene expression in mouse mammary epithelial cell lines: Dependence upon extracellular matrix and cell type

Journal Article · Tue Sep 01 04:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90105-4· OSTI ID:6530901

Medina, D; Oborn, C J ^[1]; Li, M L; Bissell, M J ^[2]

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1. Baylor College of Medicine, Houston, TX (USA)
2. Univ. of California, Berkeley (USA)

The COMMA-D mammary cell line exhibits mammary-specific functional differentiation under appropriate conditions in cell culture. The cytologically heterogeneous COMMA-D parental line and the clonal lines DB-1, TA-5, and FA-1 derived from the COMMA-D parent were examined for similar properties of functional differentiation. In monolayer cell culture, the cell lines DB-1, TA-5, FA-1, and MA-4 were examined for expression of mammary-specific and epithelial-specific proteins by an indirect immunofluorescence assay. The clonal cell lines were relatively homogeneous in their respective staining properties and seemed to represent three subpopulations found in the heterogeneous parental COMMA-D lines. None of the four clonal lines appeared to represent myoepithelial cells. The cell lines were examined for expression of {beta}-casein mRNA in the presence or absence of prolactin. The inducibility of {beta}-casein in the COMMA-D cell line was further enhanced by a reconstituted basement membrane preparation enriched in laminin, collagen IV, and proteoglycans. These results support the hypothesis that the functional response of inducible mammary cell populations is a result of interaction among hormones, multiple extracellular matrix components, and specific cell types.

• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6530901
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 172:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
BODY
CASEIN
CELL CULTURES
CELL DIFFERENTIATION
COLLAGEN
CULTURE MEDIA
CYTOLOGICAL TECHNIQUES
EPITHELIUM
GENE REGULATION
GLANDS
GLYCOPROTEINS
GONADOTROPINS
HORMONES
LH
MAMMALS
MAMMARY GLANDS
MESSENGER-RNA
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
RNA
RODENTS
SCLEROPROTEINS
TISSUES
VERTEBRATES