Conformation of methyl beta-lactoside bound to the ricin B-chain: Interpretation of transferred nuclear Overhauser effects facilitated by spin simulation and selective deuteration

Journal Article · Tue Jun 12 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00475a017· OSTI ID:6530566

Bevilacqua, V L; Thomson, D S; Prestegard, J H ^[1]

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1. Yale Univ., New Haven, CT (USA)

Spin simulation and selective deuteration have been used to aid in the interpretation of 1D transferred nuclear Overhauser effect (TRNOE) NMR experiments on ricin B-chain/ligand systems. Application of these methods has revealed a change in the conformation of deuterated methyl beta-lactoside upon binding to the ricin B-chain which results in a slight change in glycosidic torsional angels which appear to dominate in the solution conformation. The combination of simulation and experiment also shows an important sensitivity of TRNOE magnitudes to dissociation rate constants and available spin-diffusion pathways for the ricin B-chain/ligand systems under study. The sensitivity to dissociation rates allows determination of rate constants for methyl beta-lactoside and methyl beta-galactoside of 50 and 300 s-1, respectively.

• OSTI ID: 6530566
• Journal Information: Biochemistry; (USA), Vol. 29:23; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LECTINS
MOLECULAR STRUCTURE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
DEUTERIUM
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
OVERHAUSER EFFECT
TRACER TECHNIQUES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
REACTION KINETICS
RESONANCE
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques