Distribution and plasticity of immunocytochemically localized GABAA receptors in adult monkey visual cortex

Journal Article · Sun Jul 01 04:00:00 EDT 1990 · Journal of Neuroscience; (USA)

OSTI ID:6530126

Hendry, S H; Fuchs, J; deBlas, A L; Jones, E G ^[1]

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1. Univ. of California, Irvine (USA)

Immunocytochemical methods were used to reveal new details of the distribution and plasticity of GABAA receptors in the visual cortex of adult monkeys; the findings were compared with those of autoradiographic experiments involving the binding of 3H-muscimol and 3H-flunitrazepam. In both areas 17 and 18, a monoclonal antibody to the purified GABAA complex produced staining of punctate profiles in the neuropil and around cell bodies and large processes in layers I-VI. The receptor immunostaining was relatively intense in layers II-III, IVA, IVC beta, and VI; these alternated with lightly stained layers I, IVB, IVC alpha, and V. In area 18, the laminar pattern was similar except that layer IV was split into a superficial, lightly stained half and a deep, intensely stained half. In sections cut parallel to the pial surface, receptor distribution in most layers was found to be uniform. There were 3 exceptions in area 17: (1) patches of intense receptor staining were present in layers II and III; (2) a widely spaced, irregular lattice of intense staining was found in layer IVA; and (3) a much finer, regular lattice was present in layer IVC. The patches in layers II-III and the lattice in layer IVA coincided precisely with regions of intense cytochrome oxidase (CO) staining. The binding of 3H-muscimol and 3H-flunitrazepam revealed a laminar pattern that was similar in most respects, including greater ligand binding in layer IVA of area 17, but showed no evidence of the sublaminar organization in layers IVA and IVC beta. Inhomogeneities in receptor immunostaining but not ligand binding were also seen in layer III of area 18. Following a 5 or 10 d period in which intravitreal injections of TTX had silenced ganglion cell activity in one retina, GABAA receptor immunostaining in layer IVC beta was distributed in intensely stained stripes, 450-550 microns wide.

• OSTI ID: 6530126
• Journal Information: Journal of Neuroscience; (USA), Journal Name: Journal of Neuroscience; (USA) Vol. 10:7; ISSN JNRSD; ISSN 0270-6474
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMAL CELLS
ANIMALS
ANTIBODIES
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOASSAY
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISTRIBUTION
DRUGS
ENZYME IMMUNOASSAY
HYDROGEN COMPOUNDS
HYPNOTICS AND SEDATIVES
IMMUNOASSAY
KINETICS
MAMMALS
MEMBRANE PROTEINS
MONKEYS
MONOCLONAL ANTIBODIES
NERVE CELLS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
REACTION KINETICS
RECEPTORS
SOMATIC CELLS
TISSUE DISTRIBUTION
TRITIUM COMPOUNDS
VERTEBRATES