Effect of cadmium on lake water bacteria as determined by the luciferase assay of adenosine triphosphate
A firefly luciferase assay of bacterial adenosine triphosphate (ATP) was developed to measure the toxic effects of cadmium ions on aquatic organisms. Toxicity was monitored using intracellular (I/C) ATP (in micrograms per litre) as well as plate counts (colony-forming units per millilitre). The bacteria, which belonged mainly to the families Enterobacteriaceae and Pseudomonadaceae, exhibited varying degrees of resistance to up to 100 ppm cadmium when grown in a glucose-salts medium at pH 6.8. Among the organisms tested, cadmium resistance decreased in the following order: Pseudomonas vesicularis > P. aeruginosa > Enterobacter sp. > P. fluorescens > Chromobacter sp. > Serratia sp. A rise in the pH of the growth medium from 5 to 7 resulted in increased toxicity of cadmium.
- Research Organization:
- Univ. of Toronto, Toronto, Ontario
- OSTI ID:
- 6529538
- Report Number(s):
- CONF-8110103-
- Journal Information:
- Am. Soc. Test. Mater., Spec. Tech. Publ.; (United States), Vol. STP-802; Conference: American Society of Testing and Materials symposium on aquatic toxicology, St Louis, MO, USA, 15 Oct 1981
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENOSINE
TOXICITY
BACTERIA
BIOASSAY
BIOLOGICAL EFFECTS
CADMIUM
PHOSPHATES
AQUATIC ORGANISMS
LAKES
WATER POLLUTION
ELEMENTS
METALS
MICROORGANISMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
POLLUTION
RIBOSIDES
SURFACE WATERS
520200* - Environment
Aquatic- Chemicals Monitoring & Transport- (-1989)
550200 - Biochemistry
560300 - Chemicals Metabolism & Toxicology