Isomerization of all-trans-retinoids to 11-cis-retinoids in vitro

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.7.1849· OSTI ID:6529018

Bernstein, P S; Law, W C; Rando, R R

The key biochemical process of the vertebrate visual cycle required for rhodopsin regeneration, 11-cis-retinoid production from all-trans-retinoids, is shown to occur in vitro. A 600 X g supernatant from a frog retina/pigment epithelium homogenate transforms added all-trans-(/sup 3/H)retinol, in a time-dependent fashion, to a mixture of 11-cis-retinol, 11-cis-retinal, and 11-cis-retinyl palmitate. 13-cis-Retinoids are formed in only minor amounts by nonspecific processes. Studies using washed particulate fractions of the 600 X g supernatant indicate that all-trans-(/sup 3/H)retinol is isomerized to 11-cis-retinoids much more effectively than is all-trans-(/sup 3/H)retinal or all-trans-(/sup 3/H)retinyl palmitate. The 11-cis-retinoid biosynthetic activity is heat-labile, sedimentable by high-speed centrifugation, and largely found in the pigment epithelium rather than in the neural retina.

• Research Organization: Harvard Medical School, Boston, MA
• OSTI ID: 6529018
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 7; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMPHIBIANS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BODY
BODY AREAS
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
EPITHELIUM
ESTERS
EYES
FACE
FROGS
HEAD
HEXADECANOIC ACID
ISOMERIZATION
KINETICS
LABELLED COMPOUNDS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PIGMENTS
PROTEINS
REACTION KINETICS
RETINA
RETINOIC ACID
RHODOPSIN
SENSE ORGANS
TIME DEPENDENCE
TISSUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMIN A
VITAMINS