Iodobenzamide for in vivo exploration of central dopamine receptors: Evaluation in animal models of supersensitivity

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90628-5· OSTI ID:6528964

Chalon, S; Guimbal, C; Guilloteau, D; Huguet, F; Schmitt, M H; Baulieu, J L; Besnard, J C ^[1]; Mayo, W ^[2]; Desplanches, G ^[3]

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1. INSERM U316, Tours (France)
2. INSERM U259, Bordeaux (France)
3. Compagnie ORIS Industrie, Gif-sur-Yvette (France)

Iodobenzamide is a promising agent to investigate D{sub 2} receptors by SPECT in living human brain. In this work, we have evaluated this radiolabeled compound in two animal models of D{sub 2} receptors supersensitivity. In the first model, rats were treated chronically with haloperidol during three weeks (S.C. injection of 0.5 mg/kg/day). One week after the last day of treatment, they were I.V. injected with {sup 125}I-IBZM. In vivo specific binding study showed a 45 percent increase of {sup 125}I-IBZM fixation in the striatum of treated rats. In a second step of experiments, animals were unilaterally lesioned by a stereotaxic injection of 6-OHDA in the substantia nigra, 23 days before receiving {sup 125}I-IBZM. Autoradiographic analysis of coronal brain sections showed a 38 percent enhancement of {sup 125}I-IBZM in vivo binding in the striatum on the lesioned side as compared to the controlateral intact side; this increase occurred in striatal lateral area. These data demonstrate that {sup 125}I-IBZM is convenient to detect alterations of dopamine D{sub 2} receptors in vivo in the rat. Thus IBZM labelled with {sup 123}I can be a very useful imaging agent for the exploration of D{sub 2} receptors in pathological situations.

• OSTI ID: 6528964
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 47:8; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MARKERS
BIOLOGICAL PATHWAYS
BODY
BRAIN
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
COMPUTERIZED TOMOGRAPHY
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DOPAMINE
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EMISSION COMPUTED TOMOGRAPHY
EVALUATION
HOURS LIVING RADIOISOTOPES
HYDROXY COMPOUNDS
IN VIVO
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PROTEINS
RADIOISOTOPES
RADIOPHARMACEUTICALS
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SINGLE PHOTON EMISSION COMPUTED TOMOGRAPHY
SYMPATHOMIMETICS
TOMOGRAPHY
VERTEBRATES