Monoclonal antibody GB3, a new probe for the study of human basement membranes and hemidesmosomes

Journal Article · Fri May 01 00:00:00 EDT 1987 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(87)90121-2· OSTI ID:6528526

Verrando, P; Pisani, A; Serieys, N; Ortonne, J P ^[1]; Hsi, Baeli; Yeh, Changjing ^[2]

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1. UER Medecine, Nice (France)
2. INSERM U210, Nice (France)

A monoclonal antibody, GB3, has been raised against human amnion. Not only does GB3 bind to amniotic basement membrane, but it also recognizes an antigenic structure expressed by epidermal as well as by some other human basement membranes. This antigen is synthesized (and excreted) by cultured normal human epidermal keratinocytes. It is expressed to a lesser extent by the A431 epidermoid carcinoma cell line, but is not expressed by the SV40 virus-transformed SVK14 keratinocyte cell line. In ultrastructural studies, this antigen was located in the epidermal basement membrane, both in the lamina densa and in the lamina lucida, associated with hemidesmosomes. It was identified as a protein by in vitro proteolytic cleavage studies. The radio-immunoprecipitates from cultured human keratinocytes, analyzed by SDS-PAGE, showed that GB3 recognized five polypeptides of 93.5, 125, 130, 146 and 150 kD under reducing conditions. The tissue distribution of the antigen and the molecular weights (MWs) of its constitutive polypeptides suggest that it is different from other known components of basement membranes. It may provide a biochemical marker for hemidesmosomes. Furthermore, GB3 represents an interesting and original clinical probe, since the antigenic structure recognized by GB3 is lacking in Junctional Epidermolysis Bullosa, a lethal genodermatosis in which a dermo-epidermal splitting occurs at the level of lamina lucida.

• OSTI ID: 6528526
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 170:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
ANTIGENS
AUTORADIOGRAPHY
BIOLOGICAL MARKERS
BODY
DISTRIBUTION
ELECTROPHORESIS
EPIDERMIS
EPITHELIUM
FETAL MEMBRANES
MAMMALS
MAN
MEMBRANES
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PRIMATES
PROTEINS
SKIN
TISSUE DISTRIBUTION
TISSUES
VERTEBRATES