Acquisition of telomere repeat sequences by transfected DNA integrated at the site of a chromosome break

Journal Article · Mon Feb 01 04:00:00 EST 1993 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.13.2.977· OSTI ID:6527981

Murnane, J P; Yu, Lohchung ^[1]

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1. Univ. of California, San Francisco (United States)

Rearrangement of the human genome is an important element in both cancer biology and genetic disease. Rearrangements that have been observed include deletions, translocations, chromosome breakage or loss, and gene amplification. Transfection of the DNA into mammalian cells can created instability in the genome. The characterization of DNA rearrangement associated with transfected DNA may provide information about the general mechanisms involved in genomic instability. This genomic instability is an important aspect of tumor cell progression. This research examines chromosome breakage and rearrangement that results in interstitial telomere repeat sequences within the human genome. These sequences could promote genomic instability because short repeat sequences can be recombination hotspots. Also, DNA rearrangements involving telomere repeat sequences can be associated with chromosome breaks. The introduction of telomere repeat sequences at spontaneous or ionizing radiation-induced DNA strand breaks may therefore also be a mechanism of chromosome fragmentation. 52 refs., 7 figs.

• OSTI ID: 6527981
• Journal Information: Molecular and Cellular Biology; (United States), Journal Name: Molecular and Cellular Biology; (United States) Vol. 13:2; ISSN 0270-7306; ISSN MCEBD4
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMOSOMAL ABERRATIONS
CHROMOSOME LOSSES
CHROMOSOMES
COSMIDS
DNA HYBRIDIZATION
DNA REPAIR
DNA SEQUENCING
HYBRIDIZATION
LOSSES
MUTATIONS
REPAIR
RFLPS
STRAND BREAKS
STRUCTURAL CHEMICAL ANALYSIS