Analysis of the albino-locus region of the mouse. IV. Characterization of 34 deficiencies

Journal Article · Mon Mar 01 04:00:00 EST 1982 · Genetics; (United States)

OSTI ID:6527214

Russell, L B; Montgomery, C S; Raymer, G D

Thirty-four independent nonviable c-locus mutations (types c/sup al/, albino lethal and c/sup as/, albino subvital), derived from radiation experiments, were tested for invovlvement of nearby markers tp, Mod-2, sh-1, and Hbb: 10, 22, and 2 involved, respectively, none of these markers, Mod-2 alone, and Mod-2 plus sh-1. When classified on this basis, as well as according to developmental stage at which homozygotes die, and by limited complementation results, the 34 independent mutations fell into 12 groups. From results of a full-scale complementation grid of all 435 possible crosses among 30 of the mutations, we were able to postulate an alignment of eight functional units by which the 12 groups fit a linear pattern. Abnormal phenotypes utilized in the complementation study were deaths at various stages of prenatal or postnatla development, body weight, and reduction or absence of various enzymes. Some of these phenotypes can be separated (e.g., glucose-6-phosphatase deficiency and neonatal death). - We conclude that all of the nonviable albino mutations are deficiencies overalpping at c, and ranging in size from < 2cM to 6 to 11 cM. The characterization of this array of deficiencies should provide useful tools for gene-dosage studies, recombinant-DNA fine-structure analyses, etc. Since many of the combinations of lethals produce viable albino animals that resemble the standard c/c type, we conclude (1) that the c locus contains no sites essential for survival, and (b) that viable nonalbino c-locus mutations (c/sup xv/) are the result of mutations within the c cistron. Viable albinos (c/sup av/, the majority of radiation-induced c-locus mutations) may be intracistronic mutations or very small deficiencies.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6527214
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 100; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BEAMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
DATA
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FETUSES
GAMETES
GENE MUTATIONS
GENES
GENETIC EFFECTS
GENETIC MAPPING
GENETIC RADIATION EFFECTS
GENETICS
GERM CELLS
INFORMATION
IONIZING RADIATIONS
LETHAL GENES
MAMMALS
MAPPING
MICE
MORTALITY
MUTANTS
MUTATIONS
NEONATES
NEUTRON BEAMS
NUCLEON BEAMS
NUMERICAL DATA
OOCYTES
PARTICLE BEAMS
PHENOTYPE
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SPERMATOZOA
VERTEBRATES
X RADIATION