Structure of newly synthesized (/sup 35/S)-proteoglycans and (/sup 35/S)-proteoglycan turnover products of cartilage explant cultures from dogs with experimental osteoarthritis
The structure of newly synthesized proteoglycans from explant cultures of cartilage from joints subjected to transection of the anterior cruciate ligament (osteoarthritic) and from normal (non- or sham-operated) joints was examined. The structure of the products of proteoglycan turnover was also examined using explants of normal and osteoarthritic cartilage maintained in culture for a 48 h chase period. The findings were as follows: Newly synthesized (/sup 35/S)-proteoglycans extracted from cartilage explants from osteoarthritic joints whether examined 3 weeks, 3 months, or 6 months after surgery were larger than those from corresponding normal cartilage. This can be explained by the synthesis in osteoarthritic cartilage of abnormally long chondroitin sulfate chains on newly synthesised proteoglycans. The extracts also contained a newly formed small proteoglycan species that was unable to interact with hyaluronic acid. The proportion of this species was higher in osteoarthritic cartilage compared with normal, examined 3 weeks after surgery, but was generally absent from cartilage obtained 3 and 6 months after surgery. Compared with controls, a smaller proportion of the (/sup 35/S)-proteoglycans released into the maintenance medium of explant cultures of osteoarthritic cartilage during a 48 h chase period was able to interact with hyaluronic acid. However, although furnished with longer (/sup 35/S)-glycosaminoglycan chains, these proteoglycans were smaller than those from control explants.
- OSTI ID:
- 6526974
- Journal Information:
- J. Orthop. Res.; (United States), Journal Name: J. Orthop. Res.; (United States) Vol. 2; ISSN JORED
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BONE JOINTS
CARBOHYDRATES
CARTILAGE
CHEMISTRY
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DISEASES
DOGS
EVEN-ODD NUCLEI
HYALURONIC ACID
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MOLECULAR STRUCTURE
MUCOPOLYSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SKELETAL DISEASES
SKELETON
SULFUR 35
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES