Production of ethanol by immobilized Saccharomyces bayanus in an extractive fermentation system

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260290909· OSTI ID:6526950

Barros, M R.A.; Cabral, J M.S.; Novais, J M

An extractive fermentation system using immobilized yeast cells was developed to study the ethanol production at high sugar concentrations. Organic acids were used as extracting solvents of ethanol and their toxicity was tested in free and k-carrageenan entrapped cell preparations. Immobilization seems to protect cells against solvent toxicity, when long-chain organic acids, e.g., oleic acid, were used, probably due to steric and diffusional limitations, the free cells not being viable at high oleic acid concentrations. The entrapped cells also present a high metabolic activity than their free counterparts at high glucose concentrations. A solution of 300 g/L of glucose was totally fermented by the immobilized yeast cells, which when free cannot normally convert more than 200 g/L. In situ recovery of ethanol by oleic acid in a batch immobilized cell system led to higher ethanol productivities and to the fermentation of 400 g/L, when an oleic acid/medium ratio of 5 was used.

• Research Organization: Departamento de Energias Renovaveis, Lisboa, Portugal
• OSTI ID: 6526950
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 29:9; ISSN BIBIA
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CARBOXYLIC ACIDS
ETHANOL
EXTRACTION
FERMENTATION
FUNGI
GLUCOSE
HEXOSES
HYDROXY COMPOUNDS
IMMOBILIZED CELLS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
SEPARATION PROCESSES
SOLVENT EXTRACTION
TOXICITY
YEASTS
YIELDS