Construction of a general human chromosome jumping library, with application to cystic fibrosis

Journal Article · Fri Feb 27 00:00:00 EST 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.2950591· OSTI ID:6526282

Collins, F S; Drumm, M L; Cole, J L; Lockwood, W K; Woude, G F.V.; Iannuzzi, M C

In many genetic disorders, the responsible gene and its protein product are unknown. The technique known as reverse genetics, in which chromosomal map positions and genetically linked DNA markers are used to identify and clone such genes, is complicated by the fact that the molecular distances from the closest DNA markers to the gene itself are often too large to traverse by standard cloning techniques. To address this situation, a general human chromosome jumping library was constructed that allows the cloning of DNA sequences approximately 100 kilobases away from any starting point in genomic DNA. As an illustration of its usefulness, this library was searched for a jumping clone, starting at the met oncogene, which is a marker tightly linked to the cystic fibrosis gene that is located on human chromosome 7. Mapping of the new genomic fragment by pulsed field gel electrophoresis confirmed that it resides on chromosome 7 within 240 kilobases downstream of the met gene. The use of chromosome jumping should be applicable to any genetic locus for which a closely linked DNA marker is available.

• Research Organization: Univ. of Michigan Medical School, Ann Arbor
• OSTI ID: 6526282
• Journal Information: Science (Washington, D.C.); (United States), Vol. 235
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
GENETIC MAPPING
DNA
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
FIBROSIS
GENES
MAN
ONCOGENES
RESPIRATORY SYSTEM DISEASES
ANIMALS
CLONING
DISEASES
MAMMALS
MAPPING
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PRIMATES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550400* - Genetics
550900 - Pathology