Dose response in neutral filter elution

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577542· OSTI ID:6524016

Bloecher, D ^[1]

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1. King Faisal Specialist Hospital Research Centre, Riyadh (Saudi Arabia)

In neutral filter elution a nonlinear relationship between fraction of eluted DNA and dose is usually observed, which is often interpreted as a nonlinear induction of DNA double-strand breaks (DSBs) with dose. The conclusiveness of this hypothesis is questioned here on the basis of theoretical considerations regarding the size distribution of DNA fragments. A simple hydrodynamic model is proposed which generates the typical features of the dose response of neutral filter elution: (1) the shoulder at low doses, (2) a quasilinear correlation in an intermediate dose range, (3) a saturation at high doses, and (4) a linearization of the curve in the intermediate and higher dose range in a semilogarithmic plot. These features were derived even with the assumption of a linear induction of DSBs with dose. Thus it is demonstrated that the fraction of eluted DNA could conceivably be a nonlinear function of dose even if the induction of DSBs is directly proportional to the radiation dose.

• OSTI ID: 6524016
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 123:2; ISSN 0033-7587; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGORITHMS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
DNA
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
DOSES
FILTERS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
MATHEMATICAL LOGIC
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION DOSES
RADIATION EFFECTS
RADIOINDUCTION
RECOVERY
REPAIR
STRAND BREAKS