Gene expression, genome organization, and evolution of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6523451

Leisy, D J

The genome of the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata (OpMNPV) was mapped by examining overlapping Hindlll fragments from cosmid clones which had been constructed from partial Hindlll digests of viral DNA. Five OpMNPV cosmid clones containing fragments encompassing the entire OpMNPV genome were hybridized to blots of DNA from the multicapsid nuclear polyhedrosis virus of Autographa californica (AcMNPV). The hybridization pattern indicated that the genomes of these viruses are similarly organized. In order to examine a homologous region in greater detail, a /sup 32/P-labeled cloned DNA fragment (AcMNPV Hindlll-Q) containing one of the repeated sequences and a portion of the p10 gene from AcMNPV was used to probe Southern blots containing restriction endonuclease digests of AcMNPV DNA, and OpMNPV DNA. A single 3.6 Kb fragment, OpMNPV Hindlll-Q was hybridized. A small region of ca. 700 base-pairs, near the left end of the cloned fragment, was cross-hybridized. DNA sequencing in this region revealed an open reading frame of 279 base-pairs which had a detectable homology to the p10 gene of AcMNPV. Northern blot analysis of RNA from OpMNPV infected Orgyia leucostigma cells was used to define the temporal and spatial organization of transcripts from this region. No homology was detected between OpMNPV DNA and the repeated sequences element present on the AcMNPV Hindlll-Q fragment.

• Research Organization: Oregon State Univ., Corvallis (USA)
• OSTI ID: 6523451
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DNA
GENES
GENETIC MAPPING
HYBRIDIZATION
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAPPING
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
TRACER TECHNIQUES
VIRUSES