Effects of ultraviolet radiation on the production of epidermal derived thymocyte-activating factor/interleukin-1

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6520091

Gahring, L C

The effect of both a single exposure and multiple exposures to ultraviolet radiation (UVR) on the production and/or release of epidermal cell derived thymocyte activating factor/interleukin-1 (ETAF/IL-1) were investigated. A single exposure to UVR enhances the release of ETAF/IL-1 both in vitro and in vivo. This conclusion was based on the observation that: (1) in vitro UV-irradiation of the keratinocyte cell line elevated the release of ETAF/IL-1 on a per cell basis; (2) exposure of animals to UVR stimulated a number of in vivo biologic responses which are known to be mediated by ETAF/IL-1; and (3) ETAF/IL-1 could be detected in the serum of UVR exposed but not normal animals. Exposure of mice to UV-irradiation on a daily basis induced a desensitized state in which animals were found to be refractory to further stimulation with this inflammatory agent. A similar desensitization or tolerance was also shown to be induced by multiple intraperitoneal injections of bacterial lipopolysaccharide (LPS). Desensitization, induced by either LPS or UVR, was found to be regulated at the site of interaction between the cells capable of ETAF/IL-1 production and the exogenous inflammatory stimulus. The results indicate that the regulatory mechanisms for ETAF/IL-1 production which are employed by the keratinocyte are distinct from those regulating ETAF/Il-1 production by the macrophage.

• Research Organization: Utah Univ., Salt Lake City (USA)
• OSTI ID: 6520091
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CHEMICAL ACTIVATION
CHEMISTRY
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
IN VITRO
IN VIVO
MACROPHAGES
MAMMALS
MICE
ORGANS
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RODENTS
SKIN
SOMATIC CELLS
THYMOCYTES
TISSUES
ULTRAVIOLET RADIATION
VERTEBRATES