Structural studies of complex carbohydrates of plant cell walls: Technical progress report for the period September 1987--September 1988
Characterizing the polysaccharides that constitute the primary cell walls of plants has been a major research goal of our laboratory for many years. Our experience and the vastly improved technologies used to study these polysaccharides have resulted in a steadily increasing rate at which we are acquiring structural knowledge of the cell wall. We have recently completed projects that have identified the major structural features of the pectic polysaccharide rhamnogalacturonan I in monocots and in describing the rhamnogalacturonan I family found in dicot tissues. We have also made considerable progress toward elucidating the structure of the pectic polysaccharide rhamnogalacturonan II isolated from sycamore cell walls and completed the identification of 3-deoxy-D-lyxo-2-heptulosaric acids, a component of rhamnogalacturonan II. We have also recently completed studies on the comparison of the O-acetylation pattern of xyloglucans isolated from sycamore cell walls and xyloglucans isolated from the extracellular medium of sycamore cells grown in culture. Finally, we have completed work comparing various methods of preparing cell walls in order to optimize the yield of cell wall fractions solubilized by chemical extraction procedures. However, in spite of this substantial progress, we remain far from defining the complete primary structures of the noncellulosic polysaccharides of cell walls. 8 figs.
- Research Organization:
- Georgia Univ., Athens (USA). Complex Carbohydrate Research Center
- DOE Contract Number:
- FG09-85ER13426
- OSTI ID:
- 6518233
- Report Number(s):
- DOE/ER/13426-2; ON: DE89005911
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL WALL
CHEMICAL COMPOSITION
CHEMICAL WASTES
FRACTIONATION
POLYSACCHARIDES
STRUCTURE-ACTIVITY RELATIONSHIPS
EVALUATION
PLANTS
PROGRESS REPORT
CARBOHYDRATES
CELL CONSTITUENTS
DOCUMENT TYPES
ORGANIC COMPOUNDS
SACCHARIDES
SEPARATION PROCESSES
WASTES
550200* - Biochemistry