Eicosanoid production by peritoneal and splenic macrophages in mice depleted of bone marrow by /sup 89/Sr

Journal Article · Tue Mar 31 23:00:00 EST 1987 · Am. J. Pathol.; (United States)

OSTI ID:6516658

Shibata, Y; Bautista, A P; Pennington, S N; Humes, J L; Volkman, A

Previous studies showed that the prostaglandin-forming macrophages (M phi) induced in the spleens of CBA/J mice by intraperitoneal administration of Corynebacterium parvum (CP) could not be demonstrated following the depletion of bone marrow and blood monocytes with /sup 89/Sr. The present study compares prostaglandin E2 (PGE2), leukotriene C4 (LTC4), and LTB4 release by splenic and resident peritoneal M phi in /sup 89/Sr-treated mice and /sup 88/Sr controls following in vivo CP and in vitro incubation with zymosan, calcium ionophore A23187, or phorbol ester (PMA). Intraperitoneal administration of CP resulted in the appearance of PGE2- and LTB4-releasing M phi in the spleens of control but not /sup 89/Sr mice. The incorporation and quantitative distribution of 3H-arachidonic acid into membrane lipids, however, were comparable in test and control mice. Neither zymosan nor any of the other stimulatory agents was able to effect significant release of PGE2 in vitro. No release of LTC4 by splenic M phi was detectable under experimental or control conditions. In contrast, the capacity of resident peritoneal M phi to release PGE2, LTC4, and LTB4 was apparently unaffected by /sup 89/Sr-induced bone marrow and monocyte depletion with virtually no demonstrable elicitation. Resident peritoneal M phi removed after CP in such mice showed a dramatic decrease in PGE2 release when incubated in vitro with zymosan, A23187, or PMA. These results, taken with earlier findings, demonstrate characteristically different phenotypic expression of metabolism of certain eicosanoids by splenic M phi from the spleen and the peritoneal cavity and suggest in addition that the induction of PGE2-synthesizing M phi in the spleen by CP is dependent on either an immigrant cell originating in the bone marrow or a regulatory agent derived from a bone marrow cell.

• Research Organization: East Carolina Univ. School of Medicine, Greenville, NC
• OSTI ID: 6516658
• Journal Information: Am. J. Pathol.; (United States), Journal Name: Am. J. Pathol.; (United States) Vol. 1; ISSN AJPAA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CALCIUM
CARBOXYLIC ACIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELEMENTS
ESTERS
EVEN-ODD NUCLEI
HEMATOPOIETIC SYSTEM
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAPERITONEAL INJECTION
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIPIDS
MACROPHAGES
MAMMALS
MATERIALS
MEMBRANES
METALS
MICE
MONOCARBOXYLIC ACIDS
MONOCYTES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PERITONEUM
PHAGOCYTES
PHORBOL ESTERS
PHOSPHOLIPIDS
PROSTAGLANDINS
RADIOISOTOPES
RODENTS
SEROUS MEMBRANES
SOMATIC CELLS
SPLEEN
STRONTIUM 89
STRONTIUM ISOTOPES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
VERTEBRATES
ZYMOSAN