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A proton nuclear magnetic resonance investigation of proximal histidyl residues in human normal and abnormal hemoglobins: a probe for the heme pocket

Journal Article · · Biophys. J.; (United States)
; ;

Proton nuclear magnetic resonance spectroscopy at 250 MHz has been used to investigate the conformations of proximal histidyl residues of human normal adult hemoglobin, hemoglobin Kempsey (K145(HC2) Tyr ..lambda.. Asp), and hemoglobin McKees Rocks (K145(HC2) Tyr ..lambda.. Term) around neutral pH in H/sub 2/O at 27/sup 0/C, all in the deoxy form. Two resonances that occur between 58 and 76 ppm downfield from the water proton signal have been assigned to the hyperfine shifted proximal histidyl NH-exchangeable protons of the J and K-chains of deoxyhemoglobin. These two resonances are sensitive to the quaternary state of hemoglobin, amino acid substitutions in the J/sub 1/K/sub 2/-subunit interface and in the carboxy-terminal region of the K-chain, and the addition of organic phosphates. The experimental results show that there are differences in the heme pockets among these four hemoglobins studied. The structural and dynamic information derived from the hyperfine shifted proximal histidyl NH-exchangeable proton resonances complement that obtained from the ferrous hyperfine shifted and exchangeable proton resonances of deoxyhemoglobin over the spectral region from 5 to 20 ppm downfield from H/sub 2/O. The relationship between these findings and Perutz's stereochemical mechanism for the cooperative oxygenation of hemoglobin is discussed.

Research Organization:
Carnegie-Mellon Univ., Pittsburgh, PA
OSTI ID:
6516643
Journal Information:
Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 39:1; ISSN BIOJA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZOLES
BARYONS
CARBOXYLIC ACIDS
CONFORMATIONAL CHANGES
ELEMENTARY PARTICLES
FERMIONS
FREQUENCY RANGE
GLOBIN
HADRONS
HEME
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
MAGNETIC RESONANCE
MHZ RANGE
MHZ RANGE 100-1000
NUCLEAR MAGNETIC RESONANCE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
PROTONS
RESONANCE
STEREOCHEMISTRY