Immunoscintigraphy of human tumors transplanted in nude mice with radiolabeled anti-ras p21 monoclonal antibodies

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Journal of Nuclear Medicine; (USA)

OSTI ID:6510894

Katoh, Y; Nakata, K; Kohno, K; Shima, M; Satoh, A; Kusumoto, Y; Ishii, N; Kohji, T; Shiku, H; Nagataki, S ^[1]

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1. Nagasaki Univ. School of Medicine (Japan)

Anti-ras p21 monoclonal antibody (RASK-3) was used for immunoscintigraphy of human cancer cell lines in nude mice. Iodine-125-labeled RASK-3 was injected into nude mice with either human colon cancers (FCC-1 or BM-314) or lung cancer (KNS-62). Clear images were obtained in all three cancers 7 days after the injection of antibody. No localization of {sup 125}I-labeled control monoclonal antibody was observed. The ratio of tissue/blood radioactivity and % ID/g in the tumor were significantly higher than other organs by Day 8. The specific localization index examined by {sup 131}I-RASK-3 and {sup 125}I-control monoclonal antibody was also higher in the tumor than in other tissues. In the in vitro study, binding of RASK-3 to tumor cells increased significantly by treatment of cells with either lysolecithin or periodate-lysine-paraformaldehyde, which confirmed the intracellular localization of ras p21. The mechanism by which anti-ras p21 antibodies accumulate in tumor sites could be the necrotic changes in tumor cells or changes in membrane permeability of non-necrotic cells. These results provide a strong rationale for the utilization of ras p21 as a target antigen in the imaging of a variety of human cancers.

• OSTI ID: 6510894
• Journal Information: Journal of Nuclear Medicine; (USA), Vol. 31:9; ISSN 0161-5505
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
EXPERIMENTAL NEOPLASMS
DIAGNOSIS
LARGE INTESTINE
LUNGS
MONOCLONAL ANTIBODIES
SPECIFICITY
IODINE 125
IODINE 131
MAN
MICE
RADIOIMMUNOSCINTIGRAPHY
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PRIMATES
RADIOIMMUNODETECTION
RADIOISOTOPE SCANNING
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SCINTISCANNING
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics