Effects of 12-O-tetradecanoylphorbol-13-acetate and mezerein on epidermal ornithine decarboxylase activity, isoproterenol-stimulated levels of cyclic adenosine 3':5'-monophosphate, and induction of mouse skin tumors in vivo

Journal Article · Sat Dec 01 00:00:00 EST 1979 · Cancer Res.; (United States)

OSTI ID:6503455

Mufson, R A ^[1]; Fischer, S M; Verma, A K; Gleason, G L; Slaga, T J; Boutwell, R K

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1. Univ. of Wisconsin, Madison

The tumor promoter 12-O-tetradecanoylphorbol-13-acetate and the antileukemic agent mezerein are diterpene esters of plant origin with certain structural similarities. Both compounds, when applied topically to mouse skin, were equipotent on a molar basis in inducing hyperplasia, inflammation, and ornithine decarboxylase activity, as well as in reducing cyclic adenosine 3':5-monophosphate accumulation in response to ..beta..-adrenergic stimulation. In contrast, mezerein was much less effective as a tumor promoter; the phorbol ester at 8.5 nmol/application yielded 78-fold more tumors than did 8.5 nmol mezerein per application to similarly initiated SENCAR mice. The superiority of the phorbol ester was nearly as great in CD-1 mice. These results suggest that although the induction of hyperplasia and ornithine decarboxylase activity may be necessary components of the carcinogenic process, they are not sufficient; 12-O-tetradecanoylphorbol-13-acetate must accomplish an essential event not accomplished by mezerein.

• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6503455
• Journal Information: Cancer Res.; (United States), Vol. 39
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MICE
CARCINOGENESIS
PHORBOL ESTERS
TOXICITY
SPIN
TERPENES
CATALYSIS
CELL PROLIFERATION
COMPARATIVE EVALUATIONS
DECARBOXYLASES
IN VIVO
INFLAMMATION
METABOLISM
NEOPLASMS
ORNITHINE
AMINO ACIDS
ANGULAR MOMENTUM
ANIMALS
CARBON-CARBON LYASES
CARBOXY-LYASES
CARBOXYLIC ACIDS
CARCINOGENS
DISEASES
ENZYMES
ESTERS
LYASES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PARTICLE PROPERTIES
PATHOGENESIS
PATHOLOGICAL CHANGES
RODENTS
SYMPTOMS
VERTEBRATES
550200* - Biochemistry