Manganese peroxidase, produced by Trametes versicolor during pulp bleaching, demethylates and delignifies kraft pulp

Journal Article · Fri Jan 01 00:00:00 EST 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6501943

Paice, M G; Reid, I D; Bourbonnais, R; Archibald, F S; Jurasek, L ^[1]

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1. Pulp and Paper Research Institute of Canada, Pointe Claire, Quebec (Canada)

The white rot fungus Trametes (Coriolus) versicolor delignifies and bleaches kraft pulp. However, the process is slow compared with chemical bleaching and the cellulose is also attacked. This study attempts to determine the enzymology of fungal delignification and then applies the relevant enzymes directly to the pulp. Lignin peroxidase and manganese peroxidase (MnP) have both been implicated in lignin biodegradations. However, the researchers show that MnP is the critical enzyme. It is produced by bleaching cultures of T. versicolor; its peak production occurs at the same time as the maximum rate of fungal culture bleaching, and the manganese-and peroxide-dependent demethylation and delignification of kraft pulp occurs in vitro. 50 refs., 4 figs., 7 tabs.

• OSTI ID: 6501943
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 59:1; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BLEACHING
ENZYME ACTIVITY
DELIGNIFICATION
FUNGI
PAPER INDUSTRY
CELLULOSE
ENZYMES
IN VITRO
LIGNIN
CARBOHYDRATES
INDUSTRY
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
WOOD PRODUCTS INDUSTRY
550200* - Biochemistry
560300 - Chemicals Metabolism & Toxicology