Development of a plaque assay for Newcastle Disease virus. Memorandum report
Technical Report
·
OSTI ID:6501828
A reliable plaque assay system for virulent strains of Newcastle Disease virus (eg. Type BL, LaSota strain used in vaccines and at DRES as a viral BW simulant) utilizing a continuous monkey kidney cell line (LLC-MK2) is described. The use of a continuous cell line eliminates the added time and inconvenience of preparing primary cell cultures (eg. chick embryo fibroblasts) to quantitate the number of infectious virus particles.
- Research Organization:
- Defence Research Establishment Suffield, Ralston, Alberta (Canada)
- OSTI ID:
- 6501828
- Report Number(s):
- AD-A-178949/4/XAB; DRES-SM-1185
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL WARFARE AGENTS
BIRDS
CHICKENS
CONNECTIVE TISSUE CELLS
DISEASES
EMBRYOS
FIBROBLASTS
FOWL
INFECTIOUS DISEASES
MICROORGANISMS
PARASITES
PARTICLES
RELIABILITY
SOMATIC CELLS
VACCINES
VERTEBRATES
VIRUSES
WEAPONS
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOASSAY
BIOLOGICAL WARFARE AGENTS
BIRDS
CHICKENS
CONNECTIVE TISSUE CELLS
DISEASES
EMBRYOS
FIBROBLASTS
FOWL
INFECTIOUS DISEASES
MICROORGANISMS
PARASITES
PARTICLES
RELIABILITY
SOMATIC CELLS
VACCINES
VERTEBRATES
VIRUSES
WEAPONS