Oxidation of nitrapyrin to 6-chloropicolinic acid by the ammonia-oxidizing bacterium nitrosomonas europaea

Technical Report · Wed Jul 01 04:00:00 EDT 1992

OSTI ID:6501590

Vannelli, T; Hooper, A B

Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin (2-chloro-6-(trichloromethyl)-pyridine). Rapid oxidation of nitrapyrin (at a concentration of 10 microM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highest in the presence of 10 mM ammonia (0.8 nmol of nitrapyrin per min/mg of protein). The product of the reaction was 6-chloropicolinic acid. By the use of (18)O2, it was shown that one of the oxygens in 6-chloropicolinic acid came from diatomic oxygen and that the other came from water. Approximately 13% of the radioactivity of (2,6-(14)C) nitrapyrin was shown to bind to cells. Most (94%) of the latter was bound indiscriminately to membrane proteins. The nitrapyrin bound to membrane proteins may account for the observed inactivation of ammonia oxidation. (Copyright (c) 1992, American Society for Microbiology.)

• Research Organization: Minnesota Univ., St. Paul, MN (United States). Dept. of Genetics and Cell Biology
• OSTI ID: 6501590
• Report Number(s): PB-93-169076/XAB; CNN: EPA-R-816157-0-10; NA86AA-D-56112
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400201 -- Chemical & Physicochemical Properties
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMMONIA
BACTERIA
BIODEGRADATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
DECOMPOSITION
DIAGNOSTIC USES
GAS CHROMATOGRAPHY
HYDRAZINE
HYDRIDES
HYDROGEN COMPOUNDS
HYDROXYLAMINE
ISOTOPE APPLICATIONS
MASS SPECTROSCOPY
MEMBRANE PROTEINS
MICROORGANISMS
NITRIFICATION
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
ORGANIC COMPOUNDS
OXIDATION
PROTEINS
SEPARATION PROCESSES
SPECTROSCOPY
TRACER TECHNIQUES
USES