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Title: Simple fast micromethod for measuring enzyme activities which release tritium as tritium water

Journal Article · · Anal. Biochem.; (United States)

The entire procedure is carried out in a counting vial by mixing the reagents as a 20- to 30-microliters drop in the cap of a counting vial, incubating, quenching the reaction, and then distilling the tritium water produced into the chilled vial, in which it is assayed after the addition of scintillation solvent and a clean cap. The application of this technique to the analysis of serum transaminases is described.

Research Organization:
Brookhaven National Lab., Upton, NY
OSTI ID:
6501298
Journal Information:
Anal. Biochem.; (United States), Vol. 2
Country of Publication:
United States
Language:
English