Metabolism of 1-alkyl-2-acyl-GPC in human platelets in response to stimulation by thrombin
Journal Article
·
· Thromb. Res.; (United States)
Washed human platelets were incubated with radioactive 1-(/sup 3/H)alkyl-2-hydroxyglycero-3-phosphocholine (lyso-PAF) at 37 degrees C. (/sup 3/H)lyso-PAF was converted by platelets into (/sup 3/H)alkylacyl-GPC which was incorporated. Incorporation of radioactivity was time dependent and reached a maximum of 57 percent in one h. This formation and incorporation of (/sup 3/H)alkylacyl-GPC was inhibited (50%) by extracellular calcium (1.3 mM). Labeled platelets were treated for 5 min with either thrombin (2.5 U/ml) or saline solution. While there was no change in the saline control, thrombin induced a reduction in the content of (/sup 3/H)alkylacyl-GPC, accompanied by an increase in (3H)lyso-PAF presumably by stimulation of phospholipase A2. There was no apparent increase in radioactivity comigrating with PAF. This was probably due to the overwhelming dilution of the radioactive alkylacyl-GPC by the endogenous nonradioactive compound (ratio-1/3200). These studies suggest that human platelets can take up lyso-PAF and acylate it to alkylacyl-GPC which is susceptible to phospholipase A2 activity.
- Research Organization:
- Cardeza Foundation, Philadelphia, PA
- OSTI ID:
- 6501289
- Journal Information:
- Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 4; ISSN THBRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLESTERASES
CHEMICAL REACTIONS
COAGULANTS
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPASE
MAMMALS
MAN
MATERIALS
METABOLISM
PEPTIDE HYDROLASES
PRIMATES
RABBITS
REACTION KINETICS
SERINE PROTEINASES
THROMBIN
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ACYLATION
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLESTERASES
CHEMICAL REACTIONS
COAGULANTS
DRUGS
ENZYME ACTIVITY
ENZYMES
ESTERASES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPASE
MAMMALS
MAN
MATERIALS
METABOLISM
PEPTIDE HYDROLASES
PRIMATES
RABBITS
REACTION KINETICS
SERINE PROTEINASES
THROMBIN
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES