Diagnosis of and therapy for solid tumors with radiolabeled antibodies and immune fragments

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Cancer Treat. Rep.; (United States)

OSTI ID:6501024

Carrasquillo, J A; Krohn, K A; Beaumier, P; McGuffin, R W; Brown, J P; Hellstroem, K E; Hellstroem, I; Larson, S M

Antibodies which are directed against human tumor-associated antigens can potentially be used as carriers of radioactivity for in vivo diagnosis (radioimmunodetection) or treatment (radioimmunotherapy) of solid tumors, including colon, hepatoma, cholangiocarcinoma, and melanoma. Murine monoclonal antibodies (MOAB), produced by the hybridoma technique of Kohler and Milstein, are replacing conventional heterosera as sources of antibodies, because MOAB can be produced in large quantities as reproducible reagents with homogeneous binding properties. We have studied human melanoma using MOAB IgG and Fab fragments that recognize the human melanoma-associated antigens p97 and ''high-molecular-weight antigen''. Both antigens are found in the membrane of melanomas at much larger concentrations than in normal adult tissues. We have performed radioimmunodetection studies with whole immunoglobulin and have detected 88% of lesions greater than 1.5 cm. We have used Fab fragments for radioimmunotherapy and have found that large doses of radiolabeled antibodies (up to 342 mCi) can be repetitively given to patients without excessive end-organ toxicity. Two of three patients treated with high-dose radiolabeled antimelanoma Fab showed an effect from the treatment. Although both technical and biologic problems remain, the use of radiolabeled antibodies that are directed against tumor-associated antigens holds future promise as a new therapeutic approach to solid tumors that are resistant to conventional therapy.

• Research Organization: Veterans Administration Medical Center, Seattle, WA
• OSTI ID: 6501024
• Journal Information: Cancer Treat. Rep.; (United States), Journal Name: Cancer Treat. Rep.; (United States) Vol. 68:1; ISSN CTRRD
• Country of Publication: United States
• Language: English

Related Subjects

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
GLOBULINS
IMMUNOGLOBULINS
IMMUNOTHERAPY
MEDICINE
MONOCLONAL ANTIBODIES
NEOPLASMS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
PATIENTS
PROTEINS
RADIOLOGY
RADIOTHERAPY
THERAPY
TOMOGRAPHY
USES