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Title: Ozone effect on respiratory syncytial virus infectivity and cytokine production by human alveolar macrophages

Abstract

This study was performed to evaluate the effect of ozone (O3) exposure at 1 ppm for 2 hr on the susceptibility/resistance of adult human alveolar macrophages (AM) to infection with respiratory syncytial virus (RSV) in vitro and on RSV-induced cytokine production by the AM. AM were first exposed to O3 or to filtered air and then infected with RSV at multiplicities of infection (m.o.i.) of 0.1, 1.0, and 10. The percentage RSV-infected AM and the amount of infectious virus released by the cells were determined at Days 2 and 4 after infection. Interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF) levels in the supernatants were determined on Day 2. No difference in the percentage infected AM or in the amount of infectious RSV produced was found between control and O3-exposed cultures. However, O3-exposed AM infected with RSV at m.o.i. 1 produced less IL-1 in response to RSV infection than control AM: 63.6 pg/ml compared with 98.5 pg/ml. No difference in IL-1 was seen with m.o.i. 10. IL-6 levels were also decreased, but only after infection with m.o.i. 0.1. At this level of infection 830 pg/ml was produced by control AM as compared to 468.2 pg/ml by O3-exposed AM. TNF productionmore » was unaffected by O3 at all multiplicities of infection. Statistical analysis of the O3 effect on AM cytokine production induced by the different multiplicities, however, revealed no significant effect of O3. Based on these observations it appears unlikely that O3 alters susceptibility of AM to infection with RSV, nor does O3 dramatically alter cytokine production in response to RSV since effects on IL-1 and IL-6 secretion were only found with the lowest levels of infection which induced cytokine release.« less

Authors:
; ;  [1]
  1. (TRC Alliance Inc., Chapel Hill, NC (United States))
Publication Date:
OSTI Identifier:
6500590
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Research; (United States); Journal Volume: 60:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; OZONE; BIOLOGICAL EFFECTS; DOSE-RESPONSE RELATIONSHIPS; VIRUSES; INFECTIVITY; AIR POLLUTION; IMMUNITY; IN VITRO; MACROPHAGES; RESPIRATORY TRACT CELLS; ANIMAL CELLS; CONNECTIVE TISSUE CELLS; MICROORGANISMS; PARASITES; PHAGOCYTES; POLLUTION; SOMATIC CELLS; 560300* - Chemicals Metabolism & Toxicology; 550200 - Biochemistry

Citation Formats

Soukup, J., Koren, H.S., and Becker, S.. Ozone effect on respiratory syncytial virus infectivity and cytokine production by human alveolar macrophages. United States: N. p., 1993. Web. doi:10.1006/enrs.1993.1025.
Soukup, J., Koren, H.S., & Becker, S.. Ozone effect on respiratory syncytial virus infectivity and cytokine production by human alveolar macrophages. United States. doi:10.1006/enrs.1993.1025.
Soukup, J., Koren, H.S., and Becker, S.. Mon . "Ozone effect on respiratory syncytial virus infectivity and cytokine production by human alveolar macrophages". United States. doi:10.1006/enrs.1993.1025.
@article{osti_6500590,
title = {Ozone effect on respiratory syncytial virus infectivity and cytokine production by human alveolar macrophages},
author = {Soukup, J. and Koren, H.S. and Becker, S.},
abstractNote = {This study was performed to evaluate the effect of ozone (O3) exposure at 1 ppm for 2 hr on the susceptibility/resistance of adult human alveolar macrophages (AM) to infection with respiratory syncytial virus (RSV) in vitro and on RSV-induced cytokine production by the AM. AM were first exposed to O3 or to filtered air and then infected with RSV at multiplicities of infection (m.o.i.) of 0.1, 1.0, and 10. The percentage RSV-infected AM and the amount of infectious virus released by the cells were determined at Days 2 and 4 after infection. Interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF) levels in the supernatants were determined on Day 2. No difference in the percentage infected AM or in the amount of infectious RSV produced was found between control and O3-exposed cultures. However, O3-exposed AM infected with RSV at m.o.i. 1 produced less IL-1 in response to RSV infection than control AM: 63.6 pg/ml compared with 98.5 pg/ml. No difference in IL-1 was seen with m.o.i. 10. IL-6 levels were also decreased, but only after infection with m.o.i. 0.1. At this level of infection 830 pg/ml was produced by control AM as compared to 468.2 pg/ml by O3-exposed AM. TNF production was unaffected by O3 at all multiplicities of infection. Statistical analysis of the O3 effect on AM cytokine production induced by the different multiplicities, however, revealed no significant effect of O3. Based on these observations it appears unlikely that O3 alters susceptibility of AM to infection with RSV, nor does O3 dramatically alter cytokine production in response to RSV since effects on IL-1 and IL-6 secretion were only found with the lowest levels of infection which induced cytokine release.},
doi = {10.1006/enrs.1993.1025},
journal = {Environmental Research; (United States)},
number = ,
volume = 60:2,
place = {United States},
year = {Mon Feb 01 00:00:00 EST 1993},
month = {Mon Feb 01 00:00:00 EST 1993}
}