Development of a bioreactor for on-site remedial treatment of pollutant containers before permanent disposal. Final report
Technical Report
·
OSTI ID:650010
The report describes studies of model systems based on the surface growth of biofilm communities, with natural selection functioning to permit or inhibit the successful growth of any individual strain, with the goal of examining the dynamics of microbial community acclimation to pollutant compounds. In the model systems, microbial communities were maintained in columns having high surface area to volume ratios, which modelled natural soil columns and permitted community adaptation to a particular sole carbon-source pollutant. Flow cell experiments were also conducted to analyze the microbial biofilm communities, allowing comparisons between the community undergoing adaptation to a particular carbon source and that of the parent inoculum. Integration of scanning laser microscopy with various molecular probes enabled quantification of microbial growth, system structure, antigenicity, and natural physical and chemical heterogeneity. Combining the former methodology with community-level biochemical determinations facilitated the integrated analysis of microbial adaptation, biofilm architecture, and biofilm diversity as a function of pollutant degradative potential.
- Research Organization:
- University of Saskatchewan, Agriculture Development Fund, Regina, Saskatchewan (Canada)
- OSTI ID:
- 650010
- Report Number(s):
- MIC--98-05099/XAB
- Country of Publication:
- United States
- Language:
- English
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