Rapid blood clearance of biotinylated IgG after infusion of avidin

Journal Article · Sun Jan 01 04:00:00 EST 1989 · J. Nucl. Med.; (United States)

OSTI ID:6499556

Sinitsyn, V V; Mamontova, A G; Checkneva, Y Y; Shnyra, A A; Domogatsky, S P

The techniques of immunotherapy and radioimmunoimaging suffer from the problem of background: intravenously injected antibodies remain in the circulation much longer than it is necessary for effective binding to the target. Various approaches, including the postinjection of second antibodies, were explored to overcome the problem with some success. The phenomenon of a 100-fold more rapid blood clearance of biotinylated immunoglobulins after postinjection of an equivalent dose of avidin is described. The concentration of /sup 125/I-labeled biotinylated IgG in the circulation of rats slowly decreased to 20% of initial in 24 hr. Avidin injection at any interval during this period induced 90-95% reduction of radioactivity in blood in 15 min. Up to 70% of the radioactivity was recovered in the liver. Avidin-induced blood clearance of biotinylated immunoglobulins may find applications in immunotherapy and radio- or nuclear magnetic resonance immunoimaging.

• Research Organization: Institute of Experimental Cardiology, Moscow (USSR)
• OSTI ID: 6499556
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 30:1; ISSN JNMEA
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
AZOLES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BIOTIN
BLOOD
BLOOD-PLASMA CLEARANCE
BODY
BODY FLUIDS
CARBOHYDRATES
CARBOXYLIC ACIDS
CLEARANCE
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
GLOBULINS
GLUCOPROTEINS
GLYCOPROTEINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
IMMUNOGLOBULINS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LIVER
MAMMALS
MATERIALS
MEDICINE
NMR IMAGING
NUCLEAR MEDICINE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PROTEINS
RADIOIMMUNOLOGY
RADIOIMMUNOSCINTIGRAPHY
RADIOIMMUNOTHERAPY
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOLOGY
RADIOTHERAPY
RATS
REACTION KINETICS
RODENTS
SACCHARIDES
SCINTISCANNING
THERAPY
VERTEBRATES
VITAMIN B GROUP
VITAMINS