Pulmonary gallium uptake in rats with granulomatosis induced by complete Freund adjuvant

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148809087820· OSTI ID:6498704

Stanislas-Leguern, G; Masse, R; Jaubert, F; Chretien, J; Huchon, G

To investigate the mechanism of gallium-67 uptake in lung granulomatosis, we studied 13 rats in which lung granulomatosis was induced by injection of complete Freund adjuvant (CFA) and 14 controls. Gallium uptake was assessed in bronchoalveolar lavage fluid and lavaged lung. The cells responsible for gallium uptake were identified by latent image activation autoradiography. Gallium activity in both lavaged lungs and bronchoalveolar cells (BAC) was higher in CFA-treated animals than in controls (172,205 +/- 134,783 DPM versus 44,456 +/- 14,486 DPM +/- SD (p less than 0.05) and 40,083 +/- 16,350 DPM versus 9100 +/- 4114 DPM (p less than 0.05), respectively). In control rats, about two-thirds of total lung gallium was located in the interstitium, whereas in CFA-treated rats it was found in the mononuclear cells of lung granulomas. Gallium tracks were more numerous in the alveolar macrophages (AM) of CFA-treated rats than in control AM (28.4 +/- 10.0/field versus 8.4 +/- 3.8/field, p less than 0.001) but the number of tracks was proportional to the number of AM (52.4 +/- 18.7 versus 12.2 +/- 4.3, respectively; p less than 0.001). It is concluded that in rats with CFA-induced lung granulomatosis 1) pulmonary gallium uptake increases, 2) mononuclear cells are responsible for this uptake in both granulomas and AM, and 3) the increased uptake is due to the increased number of mononuclear cells.

• Research Organization: Universite Rene Descartes, Paris (France)
• OSTI ID: 6498704
• Journal Information: Exp. Lung Res.; (United States), Vol. 14:4
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
GALLIUM 67
UPTAKE
GRANULOMAS
DIAGNOSIS
LUNGS
AUTORADIOGRAPHY
BIOLOGICAL MODELS
LAVAGE
MACROPHAGES
MONOCYTES
RATS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GALLIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PHAGOCYTES
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics