Membrane lipid peroxidation: propagation and inhibition by antioxidants

Technical Report · Thu Jan 01 04:00:00 EST 1981

OSTI ID:6498383

Leung, H W

Peroxidation studies in microsomes and liposomes were performed to evaluate the importance of the interaction between ascorbate and ..cap alpha..-tocopherol. The peroxidation of rat liver microsomes by FeSO/sub 4/ in the presence of ascorbate was delayed compared to when NADPH replaced ascorbate as the electron donor. To further investigate the cooperation between ascorbate and vitamin E, a liposomal system containing polyunsaturated phospholipids was used. Ascorbic acid alone (30 to 100 ..mu..M) delayed peroxidation by 20, and at higher concentrations, 60 minutes. Physiological levels of vitamin E decreased peroxidation at early times but was apparently consumed during incubation. Vitamin C and vitamin E together suppressed peroxidation at early times at approximately the sum of the individual inhibitions. At longer times, the mixture was more effective than the sum of both vitamins alone. The role of glutathione and the significance of its interaction with ascorbate were studied. Glutathione was able to reduce dehydroascorbic acid, but ascorbic acid was unable to reduce oxidized glutathione disulfide. Glutathione and ascorbic acid were oxidized by NO/sub 2/ in vitro. Pulmonary levels of glutathione and ascorbic acid in guinea pigs exposed to NO/sub 2/ were lowered. After the administration of diethyl maleate, the glutathione concentration was decreased, but the ascorbic acid concentration was unaffected. Simultaneous exposure further depressed glutathione concentration, but not the ascorbic acid concentration. (ERB)

• Research Organization: Rochester Univ., NY (USA)
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 6498383
• Report Number(s): UR-3490-1952
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMALS
ASCORBIC ACID
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CHELATING AGENTS
CHEMICAL REACTIONS
DIGESTIVE SYSTEM
DRUGS
ESTERS
GLANDS
GLUTATHIONE
HYDROGEN COMPOUNDS
INHIBITION
IRON COMPOUNDS
LIPIDS
LIPOSOMES
MAMMALS
MEMBRANES
MICROSOMES
MONOCARBOXYLIC ACIDS
NITROGEN COMPOUNDS
NITROGEN DIOXIDE
NITROGEN OXIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
OXIDATION
OXIDES
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REDOX POTENTIAL
RESPIRATORY SYSTEM
RODENTS
STIMULATION
SULFATES
SULFUR COMPOUNDS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
VITAMIN E
VITAMINS