Influence of polyunsaturated fatty acid supplementation and membrane fluidity on ozone and nitrogen dioxide sensitivity of rat alveolar macrophages
The phospholipid polyunsaturated fatty acid (PUFA) content and the membrane fluidity of rat alveolar macrophages were modified dose-dependently and in different ways. This was done to study the importance of both membrane characteristics for the cellular sensitivity toward ozone and nitrogen dioxide. Cells preincubated with arachidonic acid (20:4) complexed to bovine serum albumin (BSA) demonstrated an increased in vitro sensitivity versus ozone and nitrogen dioxide. The phenomenon was only observed at the highest 20:4 concentrations tested, whereas the membrane fluidity of the 20:4-treated cells already showed a maximum increase at lower preincubation concentrations. Hence it could be concluded that the increased ozone and nitrogen dioxide sensitivity of PUFA-enriched cells is not caused by their increased membrane fluidity, resulting in an increased accessibility of sensitive cellular fatty acid moieties or amino acid residues. This conclusion receives further support from other observations. These results strongly support the involvement of lipid oxidation in the mechanism(s) of toxic action of both ozone and nitrogen dioxide in an intact cell system.
- Research Organization:
- Agricultural Univ., Wageningen, The Netherlands
- OSTI ID:
- 6497057
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 1-2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
MACROPHAGES
SENSITIVITY
NITROGEN DIOXIDE
TOXICITY
OZONE
CELL MEMBRANES
DOSE-RESPONSE RELATIONSHIPS
PHAGOCYTOSIS
PHOSPHOLIPIDS
RATS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHALCOGENIDES
CONNECTIVE TISSUE CELLS
ESTERS
LIPIDS
MAMMALS
MEMBRANES
MONOCARBOXYLIC ACIDS
NITROGEN COMPOUNDS
NITROGEN OXIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHAGOCYTES
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology